Journal: iScience

Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

doi: 10.1016/j.isci.2023.107826

Figure Lengend Snippet:

Article Snippet: Renal structures were stained with DyLight 649-labeled lens culinaris agglutinin (LCA) or fluorescein-conjugated wheat-germ agglutinin (WGA) (Cat#DL1048, Cat#FL1021, Vector Laboratories, Burlingame, CA, 1:400).

Techniques: Recombinant, Plasmid Preparation, Membrane, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Software

Journal: iScience

Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

doi: 10.1016/j.isci.2023.107826

Figure Lengend Snippet: Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled WGA lectin. (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).

Article Snippet: Kidney and left ventricle sections were then incubated overnight at 4°C with rhodamine-labelled WGA lectin (Cat#RL1022, Vector Laboratories, 1:400) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) to visualize cell membranes and stain nuclei, respectively.

Techniques: Staining, Control, Labeling, Cell Culture

Journal: iScience

Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

doi: 10.1016/j.isci.2023.107826

Figure Lengend Snippet:

Article Snippet: Kidney and left ventricle sections were then incubated overnight at 4°C with rhodamine-labelled WGA lectin (Cat#RL1022, Vector Laboratories, 1:400) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) to visualize cell membranes and stain nuclei, respectively.

Techniques: Recombinant, Plasmid Preparation, Membrane, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Software